Composite
TDC

Part:BBa_K2276001:Design

Designed by: Zhiwei Luo   Group: iGEM17_SCU_China   (2017-10-22)


T7 promotor and the coding sequneces of tryptophan decarboxylase (TDC) with N-terminal His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1015
    Illegal BamHI site found at 1682
    Illegal XhoI site found at 1674
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 639
    Illegal AgeI site found at 1272
    Illegal AgeI site found at 1521
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We optimized the codon sequnence for that the codon bias was one of the important parameter which influence the expression of TDC gene.


Source

The original sequnence of tryptophan decarboxylase (TDC) comes from GenBank (accession no.J04521). The sample used for experiments was artificially synthesized by Tsingke.

References

Park S, Kang K, Lee S W, et al. Production of serotonin by dual expression of tryptophan decarboxylase and tryptamine 5-hydroxylase in Escherichia coli[J]. Applied Microbiology & Biotechnology, 2011, 89(5):1387-94.